Double-Labelled Physarum Amoeboflagellate
Tubulin and Actin




Confocal Microscopy of a Physarum polycephalum amoeba in the process of transforming into a flagellate swimming cell. Double labelling for tubulin (green, indirect immunofluorescence) and actin (red, rhodamine-phalloidin). Imaging carried out with the BioRad MRC600 Confocal Microscope, using the A1/A2 filter set (simultaneous capture of both FITC and Rhodamine images). Upward-pointing arrow indicates anterior flagellum. Downward-pointing arrow indicates apparent co-localization (red-green overlap yields yellow) of microtubules (MTA 5) and actin at the base of the ridge, a flattened motile projection found on transforming cells. Unpublished image courtesy of Mark R. Adelman.

References:


1. Pagh, K.I. and Adelman, M.R. (1982): Identification of a Microfilament-Enriched, Motile Domain in Amoeboflagellates of Physarum polycephalum. J. Cell Science, 54:1-21.

2. Pagh, K.I. and Adelman, M.R. (1988): Assembly, Disassembly, and Movements of the Microfilament-Rich Ridge During the Amoeboflagellate Transformation in Physarum Polycephalum. Cell Motility and the Cytoskeleton, 11:223-234.

3. Adelman, M.R. (1992). Effects of Tubulozole on the RAFT in Physarum Polycephalum. Cell Biol. Int. Rep., 16:1055-1060.



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Last modified: Saturday, October 19, 1996