CULTURE AND GENETIC ANALYSIS OF
PHYSARUM POLYCEPHALUM

by

JENNIFER DEE, ROGER ANDERSON and JULIET BAILEY

CONTENTS

Introduction

Section A: Choice of Strains
	Heterothallic and apogamic strains
Genetic heterogeneity among strains
Finding the strain you need


Section B: Culturing the Vegetative Stages
	Culture methods for plasmodia
Culture methods for amoebae
Storage of stock cultures
Incubation temperatures
Changes in genetic constitution of amoebae during culture
Changes in plasmodia during culture


Section C: Developmental Changes and Cellular Transformations
	Encystment
The amoeba-flagellate transformation
The amoebal-plasmodial transition


Section D: Genetic Analysis
	Mutants
Genetic analysis of a newly-isolated mutant
Strain construction and linkage analysis
Dominance and complementation tests


Section E: DNA Transformation

References


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Last modified: Tuesday, December 22, 1998